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Polyplus-transfection SA
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STEMCELL Technologies Inc
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ImmunoTools
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ConnStem Inc
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AbCys s a
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Becton Dickinson
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ImmunoTools
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Image Search Results
Journal: PLoS ONE
Article Title: Mapping the Homodimer Interface of an Optimized, Artificial, Transmembrane Protein Activator of the Human Erythropoietin Receptor
doi: 10.1371/journal.pone.0095593
Figure Lengend Snippet: ( A ) Extracts were prepared from parental BaF3 cells or BaF3/HA-hEPOR cells expressing empty RVY-puro vector (V), EBC5-16, or TC2-3. Where indicated, cells were acutely stimulated with EPO. Samples were immunoprecipitated with anti-HA (3F10) antibody and immunoblotted with anti-phosphotyrosine antibody. Size of protein markers (in kDa) is shown on left. ( B ) Extracts from BaF3/HA-hEPOR cells expressing RVY-puro vector (V), EBC5-16, or TC2-3. Where indicated, cells were acutely stimulated with EPO or RPMI-IL-3 medium. Samples were immunoblotted for phosphorylated JAK2. Blot was reprobed for total JAK2. Size of protein markers (in kDa) is shown on left. ( C ) Extracts from the cells described in ( B ) were immunoblotted for phosphorylated STAT5. Blot was reprobed for total STAT5. Size of protein markers (in kDa) is shown on left.
Article Snippet: For erythroid colony forming assays in methylcellulose, 1×10 4 GFP + -infected cells/mL were washed in Iscove’s Modified Dulbecco’s Medium (L-glutamine, 25 mM HEPES, 3.024 g/L Na 2 CO 3 ) (Gibco) plus 2% FBS (Stem Cell Technologies) and diluted 1∶10 in methylcellulose medium (Methocult H4531, Stem Cell Technologies) containing 20 ng/mL
Techniques: Expressing, Plasmid Preparation, Immunoprecipitation